Isolation of uridine 5'-pyrophosphate glucuronic Acid pyrophosphorylase and its assay using p-pyrophosphate.
نویسندگان
چکیده
A procedure was devised to detect and assay uridine 5'-pyrophosphate (UDP)-glucuronic acid pyrophosphorylase in plant extracts. Substrates are UDP-glucuronic acid and (32)P-pyrophosphate, and the (32)P-uridine 5'-triphosphate produced is selectively adsorbed to charcoal. The charcoal adsorption procedure is a modification of that used to determine (32)P-adenosine 5'-triphosphate produced by adenosine 5'-pyrophosphate glucose pyrophosphorylase, and the modification greatly improves the retention of uridine 5'-triphosphate.
منابع مشابه
UDP-sugar pyrophosphorylase: a new old mechanism for sugar activation.
Abbreviations: aa, amino acid; AGPase, ADP-glc pyrophosphorylase; ara, arabinose; gal, galactose; galA, galacturonic acid; GALT, gal-1-P uridyltransferase; glc, glucose; glcA, glucuronic acid; man, mannose; PPi, pyrophosphate; SuSy, sucrose synthase; UAGPase, UDP-N-acetylglucosamine pyrophosphorylase; UGE, UDP-glc epimerase; UGPase, UDP-glc pyrophosphorylase; USPase, UDP-sugar pyrophosphorylase...
متن کاملThe formation of uridine diphosphate-glucuronic acid in plants. Uridine diphosphate-glucuronic acid pyrophosphorylase from barley seedlings.
UDP-glucuronic acid pyrophosphorylase was purified approximately 80-fold from barley seedlings by ammonium sulfate fractionation and column chromatography on DEAEcellulose and Sephadex G-100. The partially purified enzyme can be assayed radiochemically in either the forward or reverse direction and has K, values of 0.33 mM and 0.5 mM for n-glucuronic acid l-phosphate and UDP-D-glucuronic acid, ...
متن کاملEnzymatic Formation of Uridine Diphosphoglucuronic
Many studies have shown that, although glucose is converted to glucuronides without cleavage of the carbon chain (2--S), free glucuronic acid functions poorly, if at all, as a precursor of glucuronic acid-containing compounds in living systems (e.g. phenol or alcohol glucuronides, mucopolysaccharides) (9-l 1). An important advance in elucidating the nature of the intermediates in the biosynthes...
متن کاملSynthesis, characterization and properties of uridine 5'-( -D-apio-D-furanosyl pyrophosphate).
1. A method was developed for synthesizing UDP-apiose [uridine 5'-(alpha-d-apio-d-furanosyl pyrophosphate)] from UDP-glucuronic acid [uridine 5'-(alpha-d-glucopyranosyluronic acid pyrophosphate)] in 62% yield with the enzyme UDP-glucuronic acid cyclase. 2. UDP-apiose had the same mobility as uridine 5'-(alpha-d-xylopyranosyl pyrophosphate) when chromatographed on paper and when subjected to pap...
متن کاملStudies on cytidine diphosphate glucose pyrophosphorylase and related enzymes of Azotobacter vinelandii.
1. Formation of 11 sugar nucleotides from the respective nucleoside triphosphates and sugar l-phosphates have been demonstrated with the crude extract of Azofobacfer vinelandii. These are adenosine diphosphate glucose, cytidine diphosphate glucose, uridine diphosphate glucose, deoxythymidine diphosphate glucose, adenosine diphosphate N-acetylglucosamine, uridine diphosphate N-acetylglucosamine,...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Plant physiology
دوره 59 6 شماره
صفحات -
تاریخ انتشار 1977